Étude de métabolisme de Corynebacterium glutamicum au cours de procédés aéro-anaérobies et ses applications en génie métabolique

Abstract : The objective of this work is the study of Corynebacterium glutamicum metabolism, and of its potentialities, during an aero-anaerobic process. After a first phase during which the oxygen was supplied to favor the bacterial growth, the anaerobic phase was induced by the stopping of the oxygen supply and the decreasing of the agitation speed. In these culture conditions, lactate was the main metabolite produced. The production of this organic acid has been increased by modifying the transition time between the aerobic and the anaerobic phases. C. glutamicum 2262 was able to produce up to 27 g/l lactate during a batch process and up to 55 g/l during a fed batch process. To exploit the lactic acid synthesis pathway of C. glutamicum for ethanol production, the PDC and ADH genes from Zymomonas mobilis were expressed under the control of the endogenous promoter of ldhA, in the wild-type strain and in a ldhA-disrupted strain of C. glutamicum 2262. Although the ethanol productivities of these engineered strains were relatively low, the depletion of ldhA resulted in the increases of ethanol final concentration up to 15 times. A similar strategy was applied for the production of itaconate. As previously for the ethanol production, the final concentration of itaconate remained very low despite of some modifications of the process
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Hoang Bao Truc Khuat. Étude de métabolisme de Corynebacterium glutamicum au cours de procédés aéro-anaérobies et ses applications en génie métabolique. Alimentation et Nutrition. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0294⟩. ⟨tel-01750647⟩

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