Comparaison de la capacité de différenciation en cellules endothéliales, de deux types de cellules souches mésenchymateuses issues de la gelée de Wharton et de la moelle osseuse

Abstract : The incidence of cardiovascular disease remains a major public health problem. Despite the development of endovascular therapies, surgical treatment is necessary for many patients. The low availability of natural vessels, autologous or not, and the mechanical and biological limits of artificial substitutes, led to the use of a new domain: vascular engineering. In recent years, the concept has emerged and evolved. He could afford to offer new types of synthetic vascular substitutes and / or biological, in particular through the use of stem cells, offering interesting perspectives in the field of vascular engineering. The purpose of this study was to obtain a reliable and reproducible protocol to generate functional endothelial cells (ECs) from mesenchymal stem cells (MSCs) derived from the umbilical cord Wharton's jelly (WJ) and bone marrow (BM). Nevertheless, their differentiation into vascular cells needs a culture surface functionalization; our group demonstrated the potential use of polyelectrolyte multilayer made of poly(allylamine hydrochloride): PAH, and poly(styrene sulfonate): PSS, in promoting cells adhesion, proliferation and differentiation. Cells were cultured on (PAH-PSS)4 films or collagen type I (used as control), in the presence of angiogenic growth factors. Cells differentiation into EC was followed through the expression of endothelial markers (PCR and western blot); cell functionality was checked through their ability to incorporate acetylated LDL (Low Density Lipoprotein), to produce NO (Nitric oxide) and to express the von Willebrand factor (vWF). After 14 days of stimulation, only WJ-MSCs were able to generate functional ECs demonstrating the potential of combining WJ-MSCs and (PAH-PSS)4 films in vascular tissue engineering field
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Hassan Rammal. Comparaison de la capacité de différenciation en cellules endothéliales, de deux types de cellules souches mésenchymateuses issues de la gelée de Wharton et de la moelle osseuse. Médecine humaine et pathologie. Université de Lorraine, 2014. Français. ⟨NNT : 2014LORR0008⟩. ⟨tel-01750668⟩

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