Skip to Main content Skip to Navigation

Synthesis and characterization of antioxidant biomolecules

Abstract : An efficient solvent-free bioprocess was developed for the synthesis of DHA phenolic ester, using the lipase B from Candida antarctica. The protocol developed here led to high-level production (440 g/L) of DHA vanillyl ester (DHA-VE) that exhibits interesting application potential as food ingredient. DHA-VE was characterized by a high stability and a high radical scavenging activity towards DPPH, ABTS and hydroxyl radicals. Neuroprotective properties of DHA-VE were also demonstrated in rat primary neurons exposed to amyloid-[beta] oligomers. Enzymatic esterification of DHA with vanillyl alcohol (VA) led to increased DHA levels in erythrocytes and brain tissues of mice fed DHA-VE-supplemented diet comparing with DHA. No visible toxicity of the ester was found. Enrichment of emulsions with DHA-VE improved significantly their oxidative stability whatever the conditions of storage, showing the potential of DHA-VE to enrich various food matrices with DHA while protecting them against oxidation. The enzymatic process was applied to salmon oil as a source of omega-3 polyunsaturated fatty acids (PUFA). The total conversion of VA (50 g/L) was achieved after 24 h of reaction, leading to the production of a wide variety of esters that mirror the initial composition of the oil. The crude reaction medium recovered from salmon oil alcoholysis exhibited a high stability together with high antioxidant properties in comparison with native salmon oil. In conclusion, the approach that consists in bringing phenolic compounds and PUFA-rich lipids together within a single structure is expected to provide stable bioactive ingredients that should broaden the scope of application of omega-3 PUFAs whose health benefits are increasingly sought
Document type :
Complete list of metadata

Cited literature [211 references]  Display  Hide  Download
Contributor : Thèses UL Connect in order to contact the contributor
Submitted on : Thursday, March 29, 2018 - 12:55:20 PM
Last modification on : Thursday, January 7, 2021 - 3:41:32 PM


Files produced by the author(s)


  • HAL Id : tel-01750806, version 1


Mohamed Hussein Roby. Synthesis and characterization of antioxidant biomolecules. Food and Nutrition. Université de Lorraine, 2014. English. ⟨NNT : 2014LORR0083⟩. ⟨tel-01750806⟩



Record views


Files downloads