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Adhésion des IgG sur une surface hydrophobe : Théorie, modélisations et application à l'ELISA

Abstract : ELISA (Enzyme-Linked ImmunoSorbent Assay) are widely used analytical technologies in research and biomedical fields. Their implementation require to build antibodies thin films onto predominantly composed polystyrene surfaces. The high hydrophobicity of polystyrene ensures spontaneous and strong antibodies adhesion allowing to easily build antibodies monolayers. ELISA improvements lie most probably throughout improvements and comprehension of physico-chemical mecanisms on which antibodies immobilization on polystyrene are relied. In this way, our work explains a therozation essay emphasized by numerical modelling and experimental estimations by atomic force microscopy (AFM) and ELISA. Keeping in mind the so-called hydrophobic effect, thermodynamics of irrversible processes allows in a first time explaining reasons of antibodies adhesion on polystyrene. In a second time, numerical modelling in the field of random sequential additions model (RSA) show a way of surfaces saturation involving a strong trend to favor some antibodies orientations expected for ELISA. Finally, a RSA improvement in a RSA+R model taking into account orientational changes by the way of relaxation shows a link between deposition conditions and obtained monolayer structure. Such results seem to be strongly correlated with experimental facts
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Pierre de Thier. Adhésion des IgG sur une surface hydrophobe : Théorie, modélisations et application à l'ELISA. Biochimie, Biologie Moléculaire. Université de Lorraine, 2015. Français. ⟨NNT : 2015LORR0038⟩. ⟨tel-01751522⟩



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