Etude structurale, dynamique et fonctionnelle par résonance magnétique nucléaire de la méthionine sulfoxyde réductase A d'Escherichia coli :

Abstract : Methionine sulfoxide reductases (Msr) are a class of enzymes able to reduced back methionine sulfoxide, generated during an oxidative stress, to methionine. We performed a structural, dynamical and functional study by NMR of the reduced form and of an oxidized form of Escherichia coli MsrA. We solved the solution structures of these two forms and characterized their dynamic in solution. We also studied the non covalent enzyme/substrate complex, in order to determine local KD values, and made a preliminary study of the sulfenic acid intermediate which occurs in the catalytic cycle. All these results lead to a better knowledge of the structural and dynamical factors involved in the catalytic cycle of this class of enzyme and involved in the interaction with substrate (in specificity terms) and thioredoxin
Document type :
Theses
Complete list of metadatas

Cited literature [54 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01752522
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 1:50:14 PM
Last modification on : Tuesday, July 24, 2018 - 4:42:53 PM

File

2005_COUDEVYLLE_N.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01752522, version 1

Citation

Nicolas Coudevylle. Etude structurale, dynamique et fonctionnelle par résonance magnétique nucléaire de la méthionine sulfoxyde réductase A d'Escherichia coli :. Autre. Institut National Polytechnique de Lorraine, 2005. Français. ⟨NNT : 2005INPL110N⟩. ⟨tel-01752522⟩

Share

Metrics

Record views

16

Files downloads

14