Expression de tétraspanines et d'intégrines B1, et immunomodulation dans le cancer du poumon

Abstract : Tetraspans associate with integrins in multimolecular complexes on the surface of various cell types and are involved in numerous cell functions and notably in tumour invasion. The expression of tetraspans and [beta]1 integrins has been studied here in lung cancer on tumoral cell lines and on normal and tumoral pulmonary tissue samples. The influence of a chelator and of 4 cytokines on the expression of the molecules tested was also studied. The indirect immunofluorescence techniques used allowed flow cytometry and/or UV-light microscopy analysis. Determination of cytokines secretion patterns was obtained from different celllines by a'cytokine array technique. Our results show an important heterogeneity in the expression of the molecules studied and in the factors secreted by cells of the different cell lines, and support the involvement of CD9, CD82, CD151 and of CD29 in lung cancer. Moreover, expression of the molecules tested and adequate cell contacts seem to\ strongly rely on divalent cations availability. In conclusion, the immunomodulation of tetraspans and [bêta] 1 integrins expressed by pulmonary epithelial tumoral cells, by immunosuppressive cytokines or cytokines involved in the inflammatory and/or Th1 responses supports the major role of tumoral microenvironnement in expression ofthese molecules.
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Frédéric Massin. Expression de tétraspanines et d'intégrines B1, et immunomodulation dans le cancer du poumon. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN11311⟩. ⟨tel-01754368⟩

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