Ingénierie tissulaire hépatique à partir du foie décellularisé et de cellules souches mésenchymateuses de la gelée de Wharton

Abstract : There are over 100 forms of liver diseases caused by various factors and affecting a lot of people. Unfortunately, the only treatment of a terminal liver disease is liver transplantation. However, liver transplantation often fails because of the deficit in human liver donors. Recently, a new innovative alternative for treating end-stage liver disease appears: self-built organ. In liver tissue engineering the source of cells, the decellularized liver scaffold and circular culture bioreactor, are essential factors to be taken into account. The objective of this thesis is to study two steps needed for the development of an artificial liver : cells and organ decellularization. In the first stage, we collected and characterize Wharton’s-Jelly mesenchymal stem cells (WJ-MSCs), and their differentiation potential into hepatocytes. In the second stage of the work, we developed a method for liver decellularization. We were able to get acellular scaffolds by continuous perfusion with 1% SDS and Triton X100 1%. In conclusion, this study shows the capability of WJ-MSC to be differentiated into hepatocytes and the feasibility to obtain acellular livers. That open perspectives toward the development of an artificial liver and the treatment of liver diseases
Document type :
Theses
Complete list of metadatas

https://hal.univ-lorraine.fr/tel-01754487
Contributor : Thèses Ul <>
Submitted on : Friday, March 30, 2018 - 9:50:54 AM
Last modification on : Sunday, April 1, 2018 - 1:17:00 AM

File

DDOC_T_2015_0127_YE.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01754487, version 1

Collections

Citation

Junsong Ye. Ingénierie tissulaire hépatique à partir du foie décellularisé et de cellules souches mésenchymateuses de la gelée de Wharton. Médecine humaine et pathologie. Université de Lorraine, 2015. Français. ⟨NNT : 2015LORR0127⟩. ⟨tel-01754487⟩

Share

Metrics

Record views

80

Files downloads

1050