Tests des composés de nacre sur l'activité des ostéoblastes et leur identification

Abstract : With many exceptional qualities (biocompatible and osteogenic), nacre represents a natural biomaterial as a bone substitute. However, the osteogenic compounds in nacre are not yet known. Our work aims at the identification of the osteogenic compounds in nacre. The ESM (soluble ethanol matrix) is an extract of nacre that is shown to be osteogenic. From the ESM, we have tried several approaches to target and identify these compounds. Thanks to the coupling of MC3T3-E1 cells and the human osteoarthritis osteoblasts, we demonstrated that the cationic part of the ESM is osteogenic, without interaction with the anionic part. Calcium plays a role in the osteogenic activity of the ESM. Then, we created a cell line stably expressing a plasmid containing an osteogenic reporter gene (ATDC5 pMetLuc2 ColX promoter). Thanks to this cell line, we found out that the lipids and sugars in the ESM have an osteogenic effect. The peptides precipitated by TCA are also demonstrated to be osteogenic, which have led to their partial identification by LC-MS. These results allow us to move farther and faster towards the identification of osteogenic compounds in nacre and the applications of nacre in clinical orthopaedics
Document type :
Theses
Complete list of metadatas

Cited literature [216 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01757603
Contributor : Memoires Ul <>
Submitted on : Monday, July 1, 2019 - 4:25:10 PM
Last modification on : Monday, July 1, 2019 - 4:26:47 PM

File

DDOC_T_2017_0051_ZHANG.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01757603, version 1

Collections

Citation

Ganggang Zhang. Tests des composés de nacre sur l'activité des ostéoblastes et leur identification. Sciences du Vivant [q-bio]. Université de Lorraine, 2017. Français. ⟨NNT : 2017LORR0051⟩. ⟨tel-01757603⟩

Share

Metrics

Record views

84

Files downloads

40