Mise en évidence et étude comparative des protéines de transport de fer et d'autres métaux chez Ralstonia eutropha CH34 et souches dérivées

Abstract : Rastolnia eutropha CH34, formerly Alcaligenes eutrophus CH34, was initially isolated from a decantation tank by Houba et al., in 1976. Studies on this strain revealed at least 11 heavy metals resistances, carryied by two megaplasmids : pMOL28 et pMOL30, which translate a bacteria adaptation system to highly polluted soils. Firstly, the czc resistance system, carried by pMOL30, is based on cadmium, zinc and cobalt efflux. Secondly, Rastolnia eutropha CH34 had developed a highly specific system for iron transport, based on siderophore affinity, because this element is weakly biodisponible in environment. These two mechanisms, the first one facultative, and the second one inducible but constitutive, seem to be associated. The knowledge of these two mechanism is very important to understand the way of heavy metal depollution processes. The main purpose of this study was to identify the proteins of czc system and the siderophore-iron receptor. Two Rastolnia eutropha CH34 derivates were used as references : Rastolnia eutropha 1153 (which overexpresses siderophores) and Rastolnia eutropha 104 which is plasmids-free). A 45kDa outer membrane protein was identified, by electrophoresis techniques. It seems to be the siderophore-iron receptor
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Marie-Laure Ledrich. Mise en évidence et étude comparative des protéines de transport de fer et d'autres métaux chez Ralstonia eutropha CH34 et souches dérivées. Microbiologie et Parasitologie. Université Paul Verlaine - Metz, 2001. Français. ⟨NNT : 2001METZ032S⟩. ⟨tel-01775446⟩

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