Effets du stress expérimental sur les réponses comportementale, gastrique, immunitaire et endocrinienne : implications et interactions des récepteurs opioïdes et benzodiazépiniques dans les perturbations de l'immunité cellulaire chez la souris stressée

Abstract : The main objective of our study was to investigate the importance interval separating the immunization of the application of stress in disturbances on immune, behavioral and gastric functions of mice. Our results showed that the hormonal immunity was increased after the application of stress, however, the cellular immunity was considerably decreased in the stressed animals. The stress showed tendency to decrease the locomotor and explorator activity of animals in two used tests : lignht.dark box test and the straicase test. The results also indicated that the stress significantly increased the gastric mucous lesions. In order to extend our research we have conducted a comparative and experimental study using two models of stress, social and physical stress. These experiments provide some examples of disturbances generated by these two models of stress. The two models were applied at short and long term on general behavior, cellular immunity and hormonal responses. Our results showed that the two types of stress have tendancy to decrease the number of TCD4 and to increase the circulating cells NK. On the othre hand, these disturbances didn't affect the number of T CD8 cells. Concerning the gastric response, our results showed that the inflammation of the gastric mucous is more important in animals exposed to the physical stress in comparison with the isolated animals. Concerning the behaviour, we noted that the explorer activity decreased after the application of the two stress models. The dosage of plasma catecholamines was determined using HPLC and the plasmatic corticosterone level was detected by RIA technique. We noted that the apllied physical stress considerably increased the rate of the corticosterone and also of one of the catecholamines (adrenaline and noradrenaline) compared with those animals exposed to the social stress. In our study we were also interested in investigating the role and the implication of opioïdergic and benzodiazepiniques systems on the different immunities disturbances generated by stress. Our results put more emphasis on the implication of opioids and benzodiazepines receptors on the immunosuppressor effect of the experimental stress in mice. In the second part of the work, we studied the pharmacological properties of the different plants and animal compounds such as Atropa Belladonna L., Gelsemium sempervirens L., Poumon histamine and Histaminium to induce behavioral, immune and gastric reponses. The results showed that A. belladona L. has a pharmacological profile of anxiolytic type to 9 CH, whereas G. sempervirens L. possesses immunoprotective and gastroprotective properties, particulary to doses 5 CH and 15 CH. These effects are probably associated with neurotropic, anxiolytic-like effects. P. histamine resulted in a pharmacological active effect on immune system in all tested doses. These effects of P. histamine are probably associated with its pharmacological properties on immune system with a secondary effect on neurotropic type. In the second step, we studied the pharmacological properties of G. sempervirens L., P. histamine and Histaminium on stress induced alterations in TCD4, TCD8 and NK cells. The results showed that only 9 CH of all three products, reversed stress-induced response of NK cells
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Dalila Bousta. Effets du stress expérimental sur les réponses comportementale, gastrique, immunitaire et endocrinienne : implications et interactions des récepteurs opioïdes et benzodiazépiniques dans les perturbations de l'immunité cellulaire chez la souris stressée. Médecine humaine et pathologie. Université Paul Verlaine - Metz, 2001. Français. ⟨NNT : 2001METZ033S⟩. ⟨tel-01775480⟩

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