Elimination du phosphore des eaux résiduaires par voie biologiques : activité de la polyphosphate-kinase : utilisation d'une phase acidogène en amont d'un système de type "Phoredox modifié"

Abstract : To control eutrophication in the natural water bodies implies purifying waste waters before they are released into the environment. This thesis contributes to the research on the removal by bacteria of carbon, nitrogen and phosphorus from municipal waste waters. Two points have been studied. In the first part of this work, the phosphorus metabolism of bacteria was investigated, and the activity of the enzyme polyphosphate kinase was measured. In the second part, an acidogenic reactor was studied, ant its influence on the phosphorus removal in a modified phoredox-type bench scale pilot was determined. A method for measuring polyphosphate kinase activity has been developed for acinetobacter calcoaceticus. The method was applied to a number of bacterial strains and to activated sludge samples. A constitutive polyphosphate kinase activity (between 0.36 and 4.8 nmol P.min-1.mg-1 prot.) was found in some bacterial strains. In some cases, polyphosphote kinase activity was induced by anaerobiosis. The polyphosphate kinase activity of all activated sludges examined was low (<0.1 nmoles P.min-1.mg-1prot.). Experiments on a bench scale acidogenic system showed that acetate was the only volatile fatty acid produced in measurable quantities. The quantity of acetate produced mainly depends on the quantity of organic carbon in the influent. Acetate production kinetics were measured during batch experiments. The initial production was about 0.2 mg acetate.g-1ss.min-1. Improvement in efficiency of phosphorus removal in a modified phoredox pilot upon coupling after the acidogenic reactor (1 to 2.5 h hydraulic retention time) was minimal
Complete list of metadatas

Cited literature [10 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01775716
Contributor : Administrateur Du Ccsd <>
Submitted on : Tuesday, April 24, 2018 - 3:40:56 PM
Last modification on : Friday, June 29, 2018 - 1:16:25 AM
Long-term archiving on : Wednesday, September 19, 2018 - 8:29:26 AM

Identifiers

  • HAL Id : tel-01775716, version 1

Collections

Citation

Johan t'Seyen. Elimination du phosphore des eaux résiduaires par voie biologiques : activité de la polyphosphate-kinase : utilisation d'une phase acidogène en amont d'un système de type "Phoredox modifié". Génie chimique. Université Paul Verlaine - Metz, 1986. Français. ⟨NNT : 1986METZ030S⟩. ⟨tel-01775716⟩

Share

Metrics

Record views

29

Files downloads

22