Etude de biocapteurs, à algues immobilisées pour le contrôle des milieux hydriques

Abstract : Algal biosensors were investigated for use in continuous monitoring pollution of aquatic systems. Coupling biological systems with electrochemical techniques has resulted in the development of biosensors that offer the potential of real time analysis and on ligne monitoring. Two types of algal sensors were investigated ; both allowed the monitoring of photosynthetic activity, one by measuring the electron flow in chloroplasts produced by the illuminated biocatalyst, the other by monitoring photosynthetic oxygen production. The fresh water algal species : Chlorella vulgaris, Selenastrum capricornutum, Scenedesmus subspicatus and the marine algal species : Dunaliella bioculata, Skeletonema costatum, Phaedactylum tricornutum were used in these experiments. Sensivity to herbicides was found very similar for the two sensors. But oxygen electrode based sensor, according to its simplicity and its longer operationnal life, appeared to be more appropriate for toxic pollutant detection. Oxygen electrode based sensor has been patented. At the present time, we study a new prototype to be applied in field
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Pascal Pandard. Etude de biocapteurs, à algues immobilisées pour le contrôle des milieux hydriques. Sciences agricoles. Université Paul Verlaine - Metz, 1992. Français. ⟨NNT : 1992METZ013S⟩. ⟨tel-01775972⟩

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