Skip to Main content Skip to Navigation
Theses

Films multicouches à base de polyelectrolytes biodégradables : caractérisation physico-chimique et interaction avec des cellules

Abstract : ln the field of the biomaterials, the films multilayer of polyelectrolytes represent a new possible way of modification of surface: These films are built by successive adsorption of polyanions and polycations. Within the framework of this study, we worked on various types of films to characterize their physico-chemical properties by optical waveguide Iight spectroscopy, quartz crystal microbalance, confocal microscopy and atomic force microscopy. Other share, we have evaluated the interactions of the cells with films. A first part of my work consisted in characterizing films worked out containing poly(LLysine)and of poly(L-Glutamique acid). Several parameters of constructions were tested(pH, salt rate, numbers of layers deposited...). The adsorption of serum proteins on films was also evaluated, as cellular adhesion in the short run by experiments of micromanipulation. In a second part, we focused ourselves on films mullilayer containing polysaccharides, in particular chitosan and hyaluronan. Alter having characterized the growth and the structure of these films, we then studied the cellular and bacterial adhesion. We in particular highlighted that a stiffness offilms changes significantly
Document type :
Theses
Complete list of metadata

https://hal.univ-lorraine.fr/tel-01776031
Contributor : Administrateur Du Ccsd <>
Submitted on : Tuesday, April 24, 2018 - 3:50:25 PM
Last modification on : Monday, May 25, 2020 - 10:30:17 AM
Long-term archiving on: : Wednesday, September 19, 2018 - 8:14:09 AM

File

SCD_T_2004_0251_RICHERT.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01776031, version 1

Collections

Citation

Ludovic Richert. Films multicouches à base de polyelectrolytes biodégradables : caractérisation physico-chimique et interaction avec des cellules. Biotechnologies. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN11303⟩. ⟨tel-01776031⟩

Share

Metrics

Record views

87

Files downloads

804