Etude des mécanismes de la désorption : ionisation laser assistée par matrice

Abstract : This report on the mechanisms of matrix assisted laser desorption ionization (MALDI) was realized in mass spectrometry laboratory at Metz'university. This research field appeared in the laboratory after the development of biomolecules analyses by mass spectrometry and the experience stored for fifteen years on "laser desorption". We decomposed the different states of a MALDI experiment for a better understanding of this ionization process. By using some model compounds (as peptides, nucleotides, ...) we have studied more precisely the interaction between a laser beam and the different matrices. This allow us to show the great role of small neutral molecules (e.g. carbone dioxide, ...) which are ejected in the plume after the laser pulse. Then, some derivative and complexes of cyclodextrins have been studied. This part is necessary to explain first the interaction between matrix and target molecules and secondly the dependance on the laser wavelength. Thank's to the MALDI technique we can highlight both both structural differences in intact inclusion complexes characterization
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Pascal Manuelli. Etude des mécanismes de la désorption : ionisation laser assistée par matrice. Autre [cond-mat.other]. Université Paul Verlaine - Metz, 1995. Français. ⟨NNT : 1995METZ020S⟩. ⟨tel-01776921⟩

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