Le développement embryo-larvaire du danio zébré (Brachydanio rerio) : évaluation de ce modèle pour la mesure de la toxicité des effluents et des sédiments : apports de l'analyse d'images pour les mesures biométriques de l'essai embryo-larvaire

Abstract : Effluent and sediment toxicity can be measured by a great number of bioassays in laboratory conditions. The reliability of the embryo-larval stage test using zebra danio (brachydanio rerio) was evaluated for effluent and sediment samples using several methodological improvements. We determined the tolerance range of this organism to different physico-chemical parameters (pH, dissolved oxygen, hardness, un-ionized ammonia) in order to evaluate their contribution to the toxicity noticed during the tests. The advantages of using an image analysis technique in order to count and measure larvae and detect gross morphological deformities were also assessed. After testing different industrial and municipal wastewaters, the minimum toxic concentrations detected by the embryo-larval test on zebra danio were greater but in the same order of magnitude as the ones obtained by two chronic bioassays on Selenastrum capricornutum and/or ceriodaphnia dubia. The sensitivity of the "fish" model to un-ionized ammonia and a teratogenic compound (dibutylphthalate) often found in municipal wastewaters justifies its integration into a battery of bioassays. Furthermore, we found the control values of the toxic end-points to be homogeneous among the embryo-larval stage tests. On the other hand, this test showed a lower relevance to measure sediment toxicity in comparison to the Microtox test (photobacterium phosphoreum) applied on whole sediment
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Stéphane Naudin. Le développement embryo-larvaire du danio zébré (Brachydanio rerio) : évaluation de ce modèle pour la mesure de la toxicité des effluents et des sédiments : apports de l'analyse d'images pour les mesures biométriques de l'essai embryo-larvaire. Sciences agricoles. Université Paul Verlaine - Metz, 1996. Français. ⟨NNT : 1996METZ009S⟩. ⟨tel-01777112⟩

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