Mesure in situ de l'induction du cytochrome P450 1A chez des cyprinidés d'eau douce : optimisation de l'interprétation

Abstract : Biological assessment is useful in addition to conventional chemical analyses to monitor the water quality. Biochemical markers are early warning systems of the exposition of aquatic organisms to pollutants. Among them, the measurement of the fish EROD induction enables the detection of PAHs, PCBs and dioxines. The biomarker was used as a fitted tool in numerous limited field studies. Yet, in a large scale biomonitoring perspective, it is necessary to determine baseline values in sentinel fish species as well as to precisely quantify the biotic and abiotic sources of variability. Cyprinids were thus sampled in the Rhône river watershed in low polluted and in polluted areas. Reference baseline EROD activities were calculated for three species : barbel, chub and gudgeon. The data from the polluted sites were related to contamination and female sexual maturity. In the same way, laboratory experiments provided information on the effect of the level of the inducer, the influence of the sexual maturity as well as the sensitivity and reversibility of the EROD induction. The immunodetection of the cytochrome P450 1A protein provided better assessment of results where an inhibition effect could have occured. With all results, a classification of the EROD levels was proposed through statistical analysis of the EROD distributional properties. Both measurement and interpretation of the EROD biomarker are then mature enough to begin a normalisation procedure in a biomonitoring perspective
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Patrick Flammarion. Mesure in situ de l'induction du cytochrome P450 1A chez des cyprinidés d'eau douce : optimisation de l'interprétation. Sciences agricoles. Université Paul Verlaine - Metz, 1997. Français. ⟨NNT : 1997METZ049S⟩. ⟨tel-01777228⟩

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