La greffe orthotopique de biopsies de gliomes malins humains chez la souris nude : un modèle pré-clinique général de recherches en neuro-oncologie

Abstract : Human glioblastomas grafts onto nude mice brains allow a brain tumor model that closely ressemble the human situation with the exception of their microvascular bed. Chimerism sludies by in situ hybridisation coupled to immunohistochemislry allows unequivocal identification of tumor cells from reactive gliosis and vasculature of the mouse host brain. As such, this method provides a model for the analysis of the tumor cells differentiation, kinetic and migration pattern inside the parenchyma of the mouse host brain.With this model, the ability to measure the characteristics of the tumors noninvasively using NMR technique such as MRI and 31 P NMR Spectroseopy and to compare with gold standard histological techniques has been validated. Cell distribution studies of phosphorothioate oligonucleotides have demonstrated the usefulness of such a model for antisens therapy strategies in humans. The results obtained open, without ethical limitations, numerous possibilities for direct in vivostudy of experimental human gliomas.
Mots-clés : Oligonucléotides Gliome
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Theses
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Laurent Antunes. La greffe orthotopique de biopsies de gliomes malins humains chez la souris nude : un modèle pré-clinique général de recherches en neuro-oncologie. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2002. Français. ⟨NNT : 2002NAN10329⟩. ⟨tel-01777291⟩

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