Minéralisation trachéale : mécanismes cellulaires et moléculaires dans le modèle de la souris

Abstract : The trachea is a very complex structure of the respiratory tract, composed of C-shaped cartilaginous rings, made of hyaline cartilage, and muscular bands, made of smooth muscle cells, conferring rigidity and compliance to the windpipe, respectively. In contrast to other intensely studied cartilages such as the ones found in the developing growth plate and in the adult joints or smooth muscle cells from the vasculature, very little information is available on the development of the tracheal cartilage and smooth muscle tissues and on their innate propensity to mineralize, although calcification of the trachea is a common finding in the elderly population and also a rare manifestation of pathologic conditions. In this context, this PhD work sought to better characterized the poorly studied tracheal cartilage and smooth muscle tissue and understand the molecular mechanisms regulating tracheal mineralization that has been unexplored so far. We tackle these questions in the mouse model. Setting up a novel in-vitro culture of tracheal cells, we demonstrated that tracheal chondrocytes and smooth muscles cells are prone to mineralize when treated with high level of Pi, through different molecular mechanisms. In parallel, we found that in vivo mineralization of the trachea only happens in the cartilaginous rings, as early as 30 days after birth. Histological and molecular evidence suggest that tracheal mineralization occurs through a BMP-dependent rostro-caudal progression
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Lina Tabcheh. Minéralisation trachéale : mécanismes cellulaires et moléculaires dans le modèle de la souris. Médecine humaine et pathologie. Université de Lorraine, 2014. Français. ⟨NNT : 2014LORR0341⟩. ⟨tel-02075324⟩

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