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Adhésion, inactivation et persistance des adénovirus dans des biofilms d'eau potable

Abstract : Although drinking water outbreaks due to enteric viruses are scarce, they highlight their ability to spread and persist in drinking water systems. There is a lack of knowledge about virus behaviour into water distribution systems while this information is critical to improve treatment procedures. Among enteric viruses, adenoviruses show a high prevalence in the environment especially in drinking water, at least for their genome. In this context, the accumulation of adenovirus serotype 2 (HAdV-2) and bacteriophage PR772, used as a surrogate for adenoviruses, was studied using pilots fed with drinking water. We aimed to study virus accumulation on high-density polyethylene (HDPE) surfaces colonized or not by young drinking water biofilms and to evaluate the role of this biofilm in virus persistence during chlorine disinfection. We first developed an ICC-qPCR method to quantify infectious adenoviruses in environmental water samples. This method also improved the quantification of infectious adenoviruses at the laboratory scale. Before pilot experiments, the physicochemical characteristics of both viruses and surfaces were studied. HAdV-2 had the largest size, hydrodynamic diameter and hydrophobicity and phage PR772 showed the highest charge density. The biofilm led to a decrease in hydrophobicity but an increase in roughness. The two viruses behaved differently with a consistently higher accumulation rate for HAdV-2. The accumulation of phage PR772 was significantly reduced by the presence of a biofilm which accentuated the difference between the two viruses. When chlorination (10 mg/L) was applied on viruses either in suspension or accumulated in biofilm, HAdV-2 appeared as the most sensitive. The sensitivity of both viruses to chlorine decreased in presence of a biofilm showing its protective role against this oxidant. Our results confirm the role of hydrophobicity, particularly on the location of virus, but the charge density is probably involved in viral accumulation on surfaces as well.
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Submitted on : Thursday, April 2, 2020 - 10:41:54 AM
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  • HAL Id : tel-02529177, version 1


Maryse Iris Sedji. Adhésion, inactivation et persistance des adénovirus dans des biofilms d'eau potable. Ecologie, Environnement. Université de Lorraine, 2019. Français. ⟨NNT : 2019LORR0200⟩. ⟨tel-02529177⟩



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