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Transport d’une suspension active de bactéries en milieu confiné

Abstract : The transport properties of a suspension of motile bacteria under flow are a fundamental scientific issue in order to understand the colonisation of new environments by microorganisms. Numerous studies report the singular behaviour of these active suspensions in flow. Accumulation at the walls, collective motion, modification of the rheological behaviour are all phenomena that reveal the richness of these systems. Nevertheless, many questions remain. In this thesis, we present the effect of a flow on the transport of a suspension of motile Escherichia coli bacteria in confined geometries. Experiments were carried out in a Hele-Shaw cell to understand the influence of flow and confinement Peclet numbers on the transport of the suspension in a Poiseuille flow. The swimming properties of E. coli were characterised in a fluid at rest using two non-intrusive optical techniques: tracking and differential dynamic microscopy. In flow, these properties are strongly dependent on shear. Rheotaxis effect has been pointed out at the vicinity of a surface and favours upstream swimming; upstream migration also found in the bulk. We also show that confinement of the suspension influences the thickness of the boundary layer and, with the intensity of the flow, modifie the distribution of bacteria through the Hele-Shaw cell.
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Submitted on : Thursday, October 14, 2021 - 3:03:30 PM
Last modification on : Saturday, October 16, 2021 - 11:22:04 AM

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  • HAL Id : tel-03378189, version 1

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Axel Klein. Transport d’une suspension active de bactéries en milieu confiné. Mécanique des fluides [physics.class-ph]. Université de Lorraine, 2021. Français. ⟨NNT : 2021LORR0137⟩. ⟨tel-03378189⟩

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