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Investigation phytochimique, évaluation des activités larvicide et anti-acétylcholinestérase de différents extraits de Mercurialis annua L.

Abstract : This work reports the link between the larvicidal activity and the chemical composition of the different extracts of the male and female feet of Mercurialis annua L. a Euphorbiaceae often used in traditional medicine. The plant material used in our study is collected in four regions of Tunisia located in different bioclimatic stages: Bizerte, Jandouba, Nabeul and Beja. This study includes a first part devoted to a bibliographic synthesis. The second part is devoted to experiments and results. Indeed, we evaluated the antioxidant activity of the different extracts using DPPH tests. The phenolic compounds were identified and quantified by liquid chromatography coupled with a UV detector and mass spectrometry (LC-UV-ESI / MS). Our results showed that methanolic extracts from male plants have higher antioxidant activity (AOA) than that detected in extracts from female plants. On the other hand, male and female plant extracts from Bizerte showed the highest AOA level. For both sexes, Beja plant extracts have the lowest AOA. Certain phenolic compounds such as: narcissin, gallocatechin, rutin, epigallocatechin and epicatechin have been identified and quantified by LC-MS. On the other hand, the larvicidal activity of the various aqueous extracts of Mercurialis annua L. against the larvae of Tribolium confusum (Du Val) showed that the mortality could reach 100% after 48 hours of exposure for certain extracts, this is confirmed. by their low LC50. Furthermore, the study of the anti-acetylcholinesterase activity of the extracts tested shows that they are more potent AChE inhibitors than galantamine used as a positive control.Our study confirmed that extracts of the leaves and seeds of both sexes of Mercurialis annua L., coming from different regions of Tunisia, can therefore be exploited for other biological activities, among which the inhibitory action of AChE. Indeed, these extracts are rich in phenolic compounds, which gives them this therapeutic property against Alzheimer's disease and scavenging free radicals.
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File URL :
https://docnum.univ-lorraine.fr/ulprive/DDOC_T_2021_0155_BEN_NASR.pdf
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https://hal.univ-lorraine.fr/tel-03423273
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Submitted on : Wednesday, November 10, 2021 - 8:29:24 AM
Last modification on : Thursday, November 11, 2021 - 3:22:41 AM

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  • HAL Id : tel-03423273, version 1

Citation

Rania Ben Nasr. Investigation phytochimique, évaluation des activités larvicide et anti-acétylcholinestérase de différents extraits de Mercurialis annua L.. Chimie. Université de Lorraine; Université de Carthage (Tunisie), 2021. Français. ⟨NNT : 2021LORR0155⟩. ⟨tel-03423273⟩

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