Quinone- and nitroreductase reactions of Thermotoga maritima 3 peroxiredoxin–nitroreductase hybrid enzyme - Université de Lorraine Access content directly
Journal Articles Archives of Biochemistry and Biophysics Year : 2012

Quinone- and nitroreductase reactions of Thermotoga maritima 3 peroxiredoxin–nitroreductase hybrid enzyme

Abstract

Thermotoga maritima peroxiredoxin–nitroreductase hybrid enzyme (Prx–NR) consists of a FMN-containing nitroreductase (NR) domain fused to a peroxiredoxin (Prx) domain. These domains seem to function independently as no electron transfer occurs between them. The reduction of quinones and nitroaromatics by NR proceeded in a two-electron manner, and follows a ‘ping-pong’ scheme with sometimes pronounced inhibition by quinone substrate. The comparison of steady- and presteady-state kinetic data shows that in most cases, the oxidative half-reaction may be rate-limiting in the catalytic cycle of NR. The enzyme was inhibited by dicumarol, a classical inhibitor of oxygen-insensitive nitroreductases. The reduction of quinones and nitroaromatic compounds by Prx–NR was characterized by the linear dependence of their reactivity (log kcat/Km) on their single-electron reduction potentials E 7^1, while the reactivity of quinones markedly exceeded the one with nitroaromatics. It shows that NR lacks the specificity for the particular structure of these oxidants, except their single-electron accepting potency and the rate of electron self-exchange. It points to the possibility of a single-electron transfer step in a net two-electron reduction of quinones and nitroaromatics by T. maritima Prx–NR, and to a significant diversity of the structures of flavoenzymes which may perform the two-electron reduction of quinones and nitroaromatics
Fichier principal
Vignette du fichier
Anusevicius_2012_ABB.pdf (966.17 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-01332162 , version 1 (29-11-2017)

Identifiers

Cite

Zilvinas Anusevicius, Lina Misevičienė, Nicolas Rouhier, Jean-Pierre Jacquot. Quinone- and nitroreductase reactions of Thermotoga maritima 3 peroxiredoxin–nitroreductase hybrid enzyme. Archives of Biochemistry and Biophysics, 2012, 528 (1), pp.50-56. ⟨10.1016/j.abb.2012.08.014⟩. ⟨hal-01332162⟩
177 View
242 Download

Altmetric

Share

Gmail Facebook X LinkedIn More