A New Role for Escherichia coli DsbC Protein in Protection against Oxidative Stress

Abstract : Background: DsbC is a protein-disulfide isomerase present in the periplasm of Gram-negative bacteria. Results: We discovered that DsbC also regulates the redox state of the single cysteine residue of the l-arabinose-binding protein AraF. Conclusion: DsbC is involved in the protection of single cysteine residues against oxidative stress. Significance: This finding reveals a new link between oxidative stress protection and oxidative protein folding. We report a new function for Escherichia coli DsbC, a protein best known for disulfide bond isomerization in the periplasm. We found that DsbC regulates the redox state of the single cysteine of the l-arabinose-binding protein AraF. This cysteine, which can be oxidized to a sulfenic acid, mediates the formation of a disulfide-linked homodimer under oxidative stress conditions, preventing l-arabinose binding. DsbC, unlike the homologous protein DsbG, reduces the intermolecular disulfide, restoring AraF binding properties. Thus, our results reveal a new link between oxidative protein folding and the defense mechanisms against oxidative stress.
Type de document :
Article dans une revue
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2014, 289 (18), pp.12356-12364. 〈10.1074/jbc.M114.554055〉
Liste complète des métadonnées

Littérature citée [36 références]  Voir  Masquer  Télécharger

https://hal.univ-lorraine.fr/hal-01452720
Contributeur : Imopa Ul <>
Soumis le : vendredi 12 janvier 2018 - 13:16:55
Dernière modification le : vendredi 12 janvier 2018 - 13:34:23
Document(s) archivé(s) le : lundi 7 mai 2018 - 06:54:08

Fichier

Denoncin et al. - 2014 - A New...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Katleen Denoncin, Didier Vertommen, Isabelle S. Arts, Camille V. Goemans, Sophie Rahuel-Clermont, et al.. A New Role for Escherichia coli DsbC Protein in Protection against Oxidative Stress. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2014, 289 (18), pp.12356-12364. 〈10.1074/jbc.M114.554055〉. 〈hal-01452720〉

Partager

Métriques

Consultations de la notice

67

Téléchargements de fichiers

25