Modulation of Pleurodeles waltl DNA Polymerase mu Expression by Extreme Conditions Encountered during Spaceflight

Abstract : DNA polymerase µ is involved in DNA repair, V(D)J recombination and likely somatic hypermutation of immunoglobulin genes. Our previous studies demonstrated that spaceflight conditions affect immunoglobulin gene expression and somatic hypermutation frequency. Consequently, we questioned whether Polμ expression could also be affected. To address this question, we characterized Polμ of the Iberian ribbed newt Pleurodeles waltl and exposed embryos of that species to spaceflight conditions or to environmental modifications corresponding to those encountered in the International Space Station. We noted a robust expression of Polμ mRNA during early ontogenesis and in the testis, suggesting that Polμ is involved in genomic stability. Full-length Polμ transcripts are 8–9 times more abundant in P. waltl than in humans and mice, thereby providing an explanation for the somatic hypermutation predilection of G and C bases in amphibians. Polμ transcription decreases after 10 days of development in space and radiation seem primarily involved in this down-regulation. However, space radiation, alone or in combination with a perturbation of the circadian rhythm, did not affect Polμ protein levels and did not induce protein oxidation, showing the limited impact of radiation encountered during a 10-day stay in the International Space Station.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2013, 8 (7), 〈10.1371/journal.pone.0069647〉
Liste complète des métadonnées

Littérature citée [45 références]  Voir  Masquer  Télécharger

https://hal.univ-lorraine.fr/hal-01480625
Contributeur : Simpa Ul <>
Soumis le : mercredi 1 mars 2017 - 16:05:10
Dernière modification le : mercredi 22 novembre 2017 - 11:02:23
Document(s) archivé(s) le : mardi 30 mai 2017 - 17:34:41

Fichier

journal.pone.0069647.PDF
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Véronique Schenten, Nathan Guéguinou, Sarah Baatout, Jean-Pol Frippiat. Modulation of Pleurodeles waltl DNA Polymerase mu Expression by Extreme Conditions Encountered during Spaceflight. PLoS ONE, Public Library of Science, 2013, 8 (7), 〈10.1371/journal.pone.0069647〉. 〈hal-01480625〉

Partager

Métriques

Consultations de la notice

39

Téléchargements de fichiers

43