Discrimination between Native and Tn 6010 -Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy

Abstract : We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.
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Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2015, 59 (9), pp.5838 - 5840. 〈http://aac.asm.org/content/59/9/5838.full.pdf+html〉. 〈10.1128/AAC.00669-15〉
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Thomas Guillard, Anne-Laure Lebreil, Lars Hestbjerg Hansen, Aymric Kisserli, Sibel Berger, et al.. Discrimination between Native and Tn 6010 -Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2015, 59 (9), pp.5838 - 5840. 〈http://aac.asm.org/content/59/9/5838.full.pdf+html〉. 〈10.1128/AAC.00669-15〉. 〈hal-01481982〉

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