Discrimination between Native and Tn 6010 -Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy

We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.

Keywords

Multidrug efflux pump Escherichia-coli Guinolone resistance Extended-spectrum Plasmid prevalence Pneumoniae Olaquindox

Domains

Immunology Microbiology and Parasitology

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Antimicrob. Agents Chemother.-2015-Guillard-5838-40.pdf (467.16 Ko)

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https://hal.univ-lorraine.fr/hal-01481982

Submitted on : Friday, March 3, 2017-10:29:26 AM

Last modification on : Tuesday, April 30, 2024-3:18:04 PM

Long-term archiving on : Tuesday, June 6, 2017-1:11:07 PM

Dates and versions

hal-01481982 , version 1 (03-03-2017)

Identifiers

HAL Id : hal-01481982 , version 1
DOI : 10.1128/AAC.00669-15
PUBMED : 26124163
PUBMEDCENTRAL : PMC4538489

Cite

Thomas Guillard, Anne-Laure Lebreil, Lars Hestbjerg Hansen, Aymric Kisserli, Sibel Berger, et al.. Discrimination between Native and Tn 6010 -Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy. Antimicrobial Agents and Chemotherapy, 2015, 59 (9), pp.5838 - 5840. ⟨10.1128/AAC.00669-15⟩. ⟨hal-01481982⟩

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