On the production of glycogen by Pseudomonas fluorescens during biofilm development: an in situ study by attenuated total reflection-infrared with chemometrics

Abstract : Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy was used to monitor Pseudomonas fluorescens biofilms in situ, non-destructively, in real time, and under fully hydrated conditions. Changes accompanying the metabolic evolution of the sessile bacterial cells from the nascent biofilm monolayer to the beginning of the multilayered structure in the presence of nutrients were identified via the ATR-FTIR fingerprints of the young biofilm on the ATR crystal. The ATR-FTIR spectra were analysed by classical methods (time evolution of integrated intensities and profile evolution of specific bands), and also by a multivariate curve resolution, Bayesian positive source separation, to extract the pure component spectra and their change of concentration over time occurring during biofilm settlement. This work showed clearly the overproduction of glycogen by sessile P. fluorescens, which had not previously been described by other research groups.
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Article dans une revue
Biofouling, Taylor & Francis, 2014, 30 (6), pp.709-718. 〈10.1080/08927014.2014.915956〉
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https://hal.univ-lorraine.fr/hal-01505373
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Soumis le : mardi 11 avril 2017 - 12:04:15
Dernière modification le : jeudi 24 mai 2018 - 10:32:01

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Fabienne Quilès, Franois Humbert. On the production of glycogen by Pseudomonas fluorescens during biofilm development: an in situ study by attenuated total reflection-infrared with chemometrics. Biofouling, Taylor & Francis, 2014, 30 (6), pp.709-718. 〈10.1080/08927014.2014.915956〉. 〈hal-01505373〉

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