Catalasic activity in fish liver: improvement of the UV to visible analytic method.

Abstract : Antioxidative defenses and more especially catalasic activity (CAT) are studied in a large range of scientific research thematics. In environmental sciences, the problematic of oxidative stress is of great interest as pollutants can induce perturbations of redox homeostasis. Consequently, changes in antioxidative defenses levels in fish tissues and particularly in liver are used as potential biomarkers of pollution. In most studies, the CAT was assayed by following during 5 min the consumption of H2O2 in cytosolic buffered extracts at 240 nm (UV-method). This study proposed a development of this method in the visible, using permanganate and a 525-nm detection, which was more accurate, sensitive, and rapid. Moreover, the hepatic CAT of six different fish species [a cyclidae (Nimbochromis linni), 3 cyprinidae (Brachydanio rerio, Rutilus rutilus, Cyprinus carpio), an anguillidae (Anguilla anguilla), and a percidae (Perca fluviatilus)] was evaluated with the two protocols (UV- and KMnO4-method). The results but also the thermal optimum of the reaction and the interest of CAT as biomarker in ecotoxicology were discussed.
Type de document :
Article dans une revue
Fish Physiology and Biochemistry, Springer Verlag, 2013, 39 (4), pp.957-966. 〈10.1007/s10695-012-9754-6〉
Liste complète des métadonnées

https://hal.univ-lorraine.fr/hal-01576096
Contributeur : Urafpa Ul <>
Soumis le : mardi 22 août 2017 - 11:59:07
Dernière modification le : mardi 29 mai 2018 - 16:16:02

Lien texte intégral

Identifiants

Collections

Citation

Séverine Paris-Palacios, Laurence Delahaut, Alexis Carreras, Marielle Thomas, Sylvie Biagianti-Risbourg. Catalasic activity in fish liver: improvement of the UV to visible analytic method.. Fish Physiology and Biochemistry, Springer Verlag, 2013, 39 (4), pp.957-966. 〈10.1007/s10695-012-9754-6〉. 〈hal-01576096〉

Partager

Métriques

Consultations de la notice

53