Skip to Main content Skip to Navigation
Journal articles

Modulation of Lipid Metabolism and Spiramycin Biosynthesis in Streptomyces ambofaciens Unstable Mutants

Abstract : Streptomyces ambofaciens is prone to genetic instability involving genomic rearrangements at the extremities of the chromosomal DNA. An amplified DNA sequence (ADS205), including an open reading frame (orfPS), is responsible for the reversible loss of spiramycin production in the mutant strain NSA205 (ADS2051 Spi2). The product of orfPS is homologous to polyketide synthase systems (PKSs) involved in the biosynthesis of erythromycin and rapamycin and is overexpressed in strain NSA205 compared with the parental strain RP181110. As PKSs and fatty acid synthase systems have the same precursors, we tested the possibility that overexpression of orfPS also affects lipid metabolism in strain NSA205. This report focuses on comparative analysis of lipids in strain RP181110, the mutant strain NSA205, and a derivative, NSA228 (ADS2052 Spi1). NSA205 showed a dramatically depressed lipid content consisting predominantly of phospholipids and triacylglycerols. This lipid content was globally restored in strain NSA228, which had lost ADS205. Furthermore, strains RP181110 and NSA205 presented similar phospholipid and triacylglycerol compositions. No abnormal fatty acids were detected in NSA205.
Complete list of metadatas

Cited literature [25 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/hal-01658995
Contributor : Pierre Leblond <>
Submitted on : Friday, December 8, 2017 - 8:56:28 AM
Last modification on : Wednesday, May 27, 2020 - 7:22:02 PM

File

Appl. Environ. Microbiol.-1999...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Catherine Schauner, Annie Dary, Ahmed Lebrihi, Pierre Leblond, Bernard Decaris, et al.. Modulation of Lipid Metabolism and Spiramycin Biosynthesis in Streptomyces ambofaciens Unstable Mutants. Applied and Environmental Microbiology, American Society for Microbiology, 1999, 65 (6), pp.2730-2737. ⟨hal-01658995⟩

Share

Metrics

Record views

133

Files downloads

101