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Physical and Genetic Map of Streptococcus thermophilus A054

Abstract : The three restriction endonucleases Sfil, BssHl, and SmaI were found to generate fragments with suitable size distributions for mapping the genome of Streptococcus thermophilus A054. A total of 5, 8, and 24 fragments were produced with SfiI, BssHI, and SmaI, respectively. An average genome size of 1,824 kb was determined by summing the total fragment sizes obtained by digestions with these three enzymes. Partial and multiple digestions of genomic DNA in conjunction with Southern hybridization were used to map SfI, BssHII, and SmaI fragments. All restriction fragments were arranged in a unique circular chromosome. Southern hybridization analysis with specific probes allowed 23 genetic markers to be located on the restriction map. Among them, six rrn loci were precisely located. The area of the chromosome containing the ribosomal operons was further detailed by mapping some of the ApaI and SgrAI sites. Comparison of macrorestriction patterns from three clones derived from strain A054 revealed two variable regions in the chromosome. One was associated with the tandem rinD and rnnE loci, and the other was mapped in the region of the lactose operon.
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J. Bacteriol.-1994-Roussel-741...
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Yvonne Roussel, Mireille Pebay, Gérard Guédon, Jean-Marc Simonet, Bernard Decaris. Physical and Genetic Map of Streptococcus thermophilus A054 . Journal of Bacteriology, 1994, 176 (24), pp.7413 - 7422. ⟨10.1128/jb.176.24.7413-7422.1994⟩. ⟨hal-01659598⟩



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