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Combination of CD160 and CD200 as a useful tool for differential diagnosis between chronic lymphocytic leukemia and other mature B-cell neoplasms

Abstract : Introduction Chronic lymphocytic leukemia is usually diagnosed through the characteristic morphology/immunophenotype of the lymphocytes, but some CLL cases remain atypical resulting in diagnostic uncertainty. Methods Using flow cytometry analysis, we investigated the expression of CDs160/200 on B cells from 124 patients (82 CLL, 42 other B-cell neoplasms) and nine controls. CDs160/200 measurements were determined as a ratio of the mean fluorescence intensities of leukemic cells/controls and were considered positive when the ratios were ≥2 and 20, respectively. Results Sixty and 83% CLL expressed CDs160/200 as compared to 5% and 10% of other B-cell neoplasms, respectively. None of the controls showed CDs160/200 expressions. Combination of both markers was observed in 55% of CLL but only in 2% of other B-cell neoplasms, and absence of both markers occurred in 12% of CLL but in 86% of other B-cell neoplasms. Conclusion CDs160/200 were associated with markers of the gold standard ‘Matutes score’ and could be useful markers to differentiate atypical CLL from other B-cell neoplasms in the absence of available biopsies or cytogenetics and molecular studies.
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https://hal.univ-lorraine.fr/hal-01668412
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Submitted on : Wednesday, December 20, 2017 - 9:20:49 AM
Last modification on : Saturday, October 16, 2021 - 11:14:14 AM

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Jean-François Lesesve, Stéphanie Tardy, Birgit Frotscher, Véronique Latger-Cannard, Pierre Feugier, et al.. Combination of CD160 and CD200 as a useful tool for differential diagnosis between chronic lymphocytic leukemia and other mature B-cell neoplasms. International Journal of Laboratory Hematology, Wiley, 2015, 37 (4), pp.486-494. ⟨10.1111/ijlh.12315⟩. ⟨hal-01668412⟩

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