Highly sensitive and simple liquid chromatography assay with ion-pairing extraction and visible detection for quantification of gold from nanoparticles - Université de Lorraine Accéder directement au contenu
Article Dans Une Revue Talanta Année : 2018

Highly sensitive and simple liquid chromatography assay with ion-pairing extraction and visible detection for quantification of gold from nanoparticles

Résumé

A simple isocratic HPLC method using visible detection was developed and validated for the quantification of gold in nanoparticles (AuNP). After a first step of oxidation of nanoparticles, an ion-pair between tetrachloroaurate anion and the cationic dye Rhodamine B was formed and extracted from the aqueous media with the help of an organic solvent. The corresponding Rhodamine B was finally quantified by reversed phase liquid chromatography using a Nucleosil C18 (150 mm × 4.6 mm, 3 µm) column and with a mobile phase containing acetonitrile and 0.1% trifluoroacetic acid aqueous solution (25/75, V/V) at 1.0 mL min−1.and at a wavelength of 555 nm. The method was validated using methodology described by the International Conference on Harmonization and was shown to be specific, precise (RSD < 11%), accurate and linear in the range of 0.1 – 30.0 µM with a lower limit of quantification (LLOQ) of 0.1 µM. This method was in a first time applied to AuNP quality control after their synthesis. In a second time, the absence of gold leakage (either as AuNP or gold salt form) from nanostructured multilayered polyelectrolyte films under shear stress was assessed.
Fichier non déposé

Dates et versions

hal-01686107 , version 1 (17-01-2018)

Identifiants

Citer

Arnaud Pallotta, Valentin Philippe, Ariane Boudier, Pierre Leroy, Igor Clarot. Highly sensitive and simple liquid chromatography assay with ion-pairing extraction and visible detection for quantification of gold from nanoparticles. Talanta, 2018, 179, pp.307-311. ⟨10.1016/j.talanta.2017.11.016⟩. ⟨hal-01686107⟩
69 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More