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Highly sensitive and simple liquid chromatography assay with ion-pairing extraction and visible detection for quantification of gold from nanoparticles

Abstract : A simple isocratic HPLC method using visible detection was developed and validated for the quantification of gold in nanoparticles (AuNP). After a first step of oxidation of nanoparticles, an ion-pair between tetrachloroaurate anion and the cationic dye Rhodamine B was formed and extracted from the aqueous media with the help of an organic solvent. The corresponding Rhodamine B was finally quantified by reversed phase liquid chromatography using a Nucleosil C18 (150 mm × 4.6 mm, 3 µm) column and with a mobile phase containing acetonitrile and 0.1% trifluoroacetic acid aqueous solution (25/75, V/V) at 1.0 mL min−1.and at a wavelength of 555 nm. The method was validated using methodology described by the International Conference on Harmonization and was shown to be specific, precise (RSD < 11%), accurate and linear in the range of 0.1 – 30.0 µM with a lower limit of quantification (LLOQ) of 0.1 µM. This method was in a first time applied to AuNP quality control after their synthesis. In a second time, the absence of gold leakage (either as AuNP or gold salt form) from nanostructured multilayered polyelectrolyte films under shear stress was assessed.
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https://hal.univ-lorraine.fr/hal-01686107
Contributor : Ariane Boudier Connect in order to contact the contributor
Submitted on : Wednesday, January 17, 2018 - 8:52:48 AM
Last modification on : Friday, September 10, 2021 - 1:04:03 PM

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Arnaud Pallotta, Valentin Philippe, Ariane Boudier, Pierre Leroy, Igor Clarot. Highly sensitive and simple liquid chromatography assay with ion-pairing extraction and visible detection for quantification of gold from nanoparticles. Talanta, Elsevier, 2018, 179, pp.307-311. ⟨10.1016/j.talanta.2017.11.016⟩. ⟨hal-01686107⟩

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