Évaluation de la méthode de séquençage du génome entier comme outil pour la surveillance des infections a yersinia enterocolitica au Luxembourg

Abstract : Yersinia enterocolitica is the third most commonly reported foodborne pathogen in Europe. In Luxembourg, an excess of human cases was observed during 2012, the reasons for which remain unknown. There is currently no internationally accepted discriminatory typing method of this species for surveillance or outbreak investigations. Here we describe the use of whole genome sequencing combined with an ad hoc core genome MLST (cgMLST) scheme for the assessment of the genomic diversity of the strains collected in Luxembourg over a 5 year period (2012-2016). In addition, strains from biotype 1A, considered as non-pathogenic, were also included in this study and screened for presence of chromosomally encoded virulence genes. Our findings suggest that there is a much higher genomic diversity within biotype 1A strains than within O:3 and O:9 strains, that some small human clusters of O:3 strains are likely to have a common source and that there were small outbreaks in 2012 and in 2014 in Luxembourg. Moreover, as the MyfA peptide alleles of the non-pathogenic strains are very variable, the MyfA protein encoded might be non-functional.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/prive/BUS_M_2016_NENNIG_MORGANE.pdf
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https://hal.univ-lorraine.fr/hal-01767545
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Submitted on : Monday, April 16, 2018 - 12:12:11 PM
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Morgane Nennig. Évaluation de la méthode de séquençage du génome entier comme outil pour la surveillance des infections a yersinia enterocolitica au Luxembourg. Biotechnologies. 2016. ⟨hal-01767545⟩

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