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Master thesis

Caractérisation des extractibles de l’épicéa (Picea abies) et évaluation de leurs effets sur la croissance des microorganismes (bactéries et champignons) isolés du bois et sur leurs interactions

Abstract : Wood decay in temperate forest ecosystems is an important process to nutrient recycling. Saprotrophic fungi and bacteria play a major role in this process. However, presence of wood extracts, with some of them displaying toxicity, represent a barrier for these microorganisms. The main aim of the internship was to study the effect of heartwood extracts from Norway spruce (Picea abies) on the growth of microorganism and on their interactions. After extraction and characterization of the 5 extract fractions by GC/MS, their effect has been evaluated on 25 bacterial strains isolated from wood, on 25 bacterial strain isolated from soil, and on the white-rot fungus P. chrysosporium RP78. The results have showed that E1 fraction, mostly representing lipophilic molecules, significantly decreased the growth rate, and on the contrary, significantly increased the latency period of soil bacteria in comparison to wood bacteria. Bioactive molecules that might be involved in this inhibition could be the abietic acid, tetracosanol and dodecanol. Interaction tests showed that synergic or inhibitory interactions between microorganisms do exist in presence of wood extracts.
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http://docnum.univ-lorraine.fr/prive/BUS_M_2016_MAAROUFI_MEHDI.pdf
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https://hal.univ-lorraine.fr/hal-01767553
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Submitted on : Monday, April 16, 2018 - 12:22:12 PM
Last modification on : Monday, April 16, 2018 - 12:22:12 PM

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  • HAL Id : hal-01767553, version 1

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Mehdi Maaroufi. Caractérisation des extractibles de l’épicéa (Picea abies) et évaluation de leurs effets sur la croissance des microorganismes (bactéries et champignons) isolés du bois et sur leurs interactions. Biotechnologies. 2016. ⟨hal-01767553⟩

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