Rôles des régulations transcriptionnelles et post-transcriptionnelles dans la réponse au nitrate chez arabidopsis thaliana

Abstract : Nitrate (NO3 -), the major nitrogen (N) source for plants, is a key mineral for their proper growth. Genes encoding NO3 - transporters are tightly regulated by plants in order to adapt to a changing nutritional environment. NRT2.1 is the main component of the high affinity transport system of NO3 - in roots. During the primary nitrate response (PNR), NRT2.1 and an important number of genes involved in N metabolism are quickly induced, within minutes, after the addition of NO3 - in the medium. The aim of this work was to investigate different transcriptional and post-transcriptional molecular mechanisms involved in the PNR. It first demonstrates that SDG8, HNI9 and ARP6, different actors of chromatin dynamic, do not take part of the transcriptional regulation of the RPN, and that post-transcriptional mRNA decay, through the action of XRN exoribonucleases, does not contribute to the regulation of this response. Furthermore, this work suggests the establishment of a transcriptional memory at NRT2.1 locus in response to NO3 -. At last, this study reveals that NO3 - induction is associated with a decrease of nucleosome density at NRT2.1 locus when plants are grown on a depleted NO3 - medium
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/prive/BUS_M_2015_CHANTRENNE_JULIETTE.pdf
Complete list of metadatas

https://hal.univ-lorraine.fr/hal-01769958
Contributor : Memoires Ul <>
Submitted on : Wednesday, April 18, 2018 - 2:52:43 PM
Last modification on : Thursday, April 19, 2018 - 1:13:26 AM

Identifiers

  • HAL Id : hal-01769958, version 1

Collections

Citation

Juliette Chantrenne. Rôles des régulations transcriptionnelles et post-transcriptionnelles dans la réponse au nitrate chez arabidopsis thaliana. Sciences de l'environnement. 2015. ⟨hal-01769958⟩

Share

Metrics

Record views

45