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Master thesis

Evaluation de l'impact de différents stress chroniques sur la diversité du microbiote intestinal murin

Abstract : Intestinal microbiota is a complex ecosystem that, through constant host-microbial cross-talk, has many essential functions for our heath. Recent data suggest that host's stress could impact composition and diversity of intestinal microbiota. Involvement of intestinal dybiosis has been correlated with numerous diseases. This imbalanced microbiota was also associated with weak immune system and increased risks of infections. The aim of this study is to assess the impact of different stresses on intestinal microbial diversity with murine model through two molecular approaches. The first one is pyrosequencing 16S rRNA gene to assess the composition of microbiota. At the same time, we used real-time quantitative PCR to quantify bacterial abundance and relative abundance main gut's phyla. (Bacteroidetes and Firmicutes). These methods were applied to intra-caecal samples (n=90) from mice that were chronically stressed during 21 days as well as from control group.We compared the effect on microbiota of a psychologic chronic ultra-mild stress (CUMS) and of two hypergravity stress (mechanic stress alone or associated to psychocological stress). Characterization of microbial groups that are impacted by chronic stress could lead to a better understanding of some species roles in microbiota equilibrium. At mid-term, these results would allow to test pharmacological and nutritional approaches that could limit chronic stress-associated dysbiosis in order to avoid development or worsening of pathologies.
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Master thesis
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Submitted on : Wednesday, May 2, 2018 - 8:56:44 AM
Last modification on : Saturday, April 30, 2022 - 4:03:26 PM

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  • HAL Id : hal-01782433, version 1


Yasmine Chetouane. Evaluation de l'impact de différents stress chroniques sur la diversité du microbiote intestinal murin. Biotechnologies. 2013. ⟨hal-01782433⟩



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