Aware of the importance of provide safety blood, the World Health Organization?s (WHO) aimed to obtain all blood supplies from voluntary unpaid and regular donors by 2020 , sharing this idea the district department of health in Bogotá, aims to increase to 25% the normal volunteer blood donors as a strategy to obtain safety transfusion of the city, to 2016. The principal objective of this study is to identify intervention strategies to strengthen the culture of voluntary and habitual blood donation for Bogota?s blood banks network, In the frame of the program "Bogotá Humana" 2012 -2016. A systematic review was performed in two data bases Banque De Donnés en Santé Publique and Virtual Health Library, which includes LILACS, IBECS, MEDLINE, The Cochrane Library and SciELO. With the advice of experts two additional tools were developed to support the strengthening of the culture of regular and voluntary blood donation in Bogota. 26 studies were reviewed, and three main strategies were identified: motivational interventions, incentive interventions, remainders and knowledge interventions. The results suggest that combined interventions may improve intensions of blood donations in first and repetitive blood donors. This document develops the first stage of diagnosis and design of the interinstitutional project, the next step is the implementation of the strategies suggested in line with policy willingness.