Relationship between F-specific RNA phage genogroups, faecal pollution indicators and human adenoviruses in river water

Abstract : Recent studies have shown the increasing interest of F-specific RNA phage genotyping to identify major sources of faecal contamination in waters. This study, conducted in a river located in an urbanized watershed with recognized anthropogenic influences, was aimed at evaluating the relevance of direct phage genotyping by real-time RT-PCR. One hundred percent of positive results were obtained with a 5 mL aliquot of river water (n=31). Phage distribution was modified after cultivation, since the ratio of the two most abundant genogroups (II and I) reached 1.51 log(10) by direct RT-PCR-based method versus 0.30 log(10) after cultivation (n=8). For the first time, positive correlations between the concentrations of genogroup II, bacterial indicators and human adenoviruses were observed, which may indicate a human faecal pollution. No correlation between genogroups II and I has been revealed. The concentration of genogroup I was only correlated with water turbidity, suggesting an animal pollution coming from upstream after rainfall events. Among the microbiological parameters studied, only genogroup II/genogroup I ratio shows variations occurring in the major sources of faecal pollution.
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https://hal.univ-lorraine.fr/hal-01802419
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Submitted on : Tuesday, May 29, 2018 - 11:40:22 AM
Last modification on : Tuesday, October 9, 2018 - 3:22:04 PM

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Leslie Ogorzaly, Adeline Tissier, Isabelle Bertrand, Armand Maul, Christophe Gantzer. Relationship between F-specific RNA phage genogroups, faecal pollution indicators and human adenoviruses in river water. Water Research, IWA Publishing, 2009, 43 (5), pp.1257 - 1264. ⟨10.1016/j.watres.2008.12.011⟩. ⟨hal-01802419⟩

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