Surexpression d’un gène (sht1) codant pour une SpermidineHydroxycinnamoylTransférase dans des souches transformées de chevelus racinaires de chicorée : étude biochimique et transcriptomique

Abstract : Chicory (Cichorium intibus) has the capacity to accumulate in its roots 2 phenolic compounds such as the chlorogenic acid (CQA) and the isochlorogenic acid (di-CQA). 2 HCTs and 3 HQTs, enzymes belonging to the BAHD family, are involved in the CQA biosynthesis pathway. Phylogenetic and biochemical analyses revealed a SpermidineHydroxycinnamoyTransferase (SHT1) also belonging to the BAHD family. In order to validate the putative function of the sht1 gene, this one has been overexpressed in a transgenic hairy root lines of chicory with the Agrobacterium rhizogenes strains, 2659 and 15834. The transcriptomic analyses showed that sht1 has been well overexpressed. But biochemical analyses, didn’t have shown tricoumaroylspermidine production, also after Meja and ergosterol elicitation. Moreover, in order to understand the Agrobacterium rhizogenes transformation impact on the CQA and di-CQA production, transcriptomic analyses with qPCR have been made on two genes involved in the jasmonate signalisation pathway, seven genes involved in the hydroxycinnamic acids pathway and two genes supposed involved in the di- CQA synthesis.
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Master thesis
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Guillaume Bernard. Surexpression d’un gène (sht1) codant pour une SpermidineHydroxycinnamoylTransférase dans des souches transformées de chevelus racinaires de chicorée : étude biochimique et transcriptomique. Sciences de l'environnement. 2016. ⟨hal-01824672⟩

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