Elaboration d’un biofilm artificiel électroactif

Abstract : Electro active artificial biofilm is a structure mimicking natural biofilm with monoclonal bacterial population immobilized in film which is able to transfer electron to external environment. Bacterium’s growth and reliability could be controlled in those films. We have evaluated the interest of layer-by-layer’s assembly with bacterium (Pseudomonas Putida) and carbon nanotubes (CNT) to get such electroactive artificial biofilm. CNT are conductive nanoparticles which could improve electron transfer between biofilm and final electron acceptor. Primary experiments have shown no electroactivity with the primary assembly. Experience of flocculation confirmed that cytotoxicity of CNT was detrimental to the metabolic activity of bacteria. Adapted strategy allow us to strongly minimize this toxicity using cytochrome c or silica in the deposition protocol. Final assembly involving bacteria, cytochrome c, CNT and silica show significant electroactivity.
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Master thesis
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http://docnum.univ-lorraine.fr/public/BUS_M_2014_PINCK_STEPHANE.pdf
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Stéphane Pinck. Elaboration d’un biofilm artificiel électroactif. Microbiologie et Parasitologie. 2014. ⟨hal-01838064⟩

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