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Master thesis

Caractérisation fonctionnelle des protéines SSP1-like : approches biochimiques et physiologiques

Abstract : During the wood-decay process, Phanerochaete chrysosporium is submitted to an "extractives" induced stress. Transcriptomic analysis on P. chrysosporium in the presence of wood extracts revealed an induction of genes which encode stress response proteins and small secreted protein with unknown function. The aim of this internship was the characterization of one of these proteins (SSP1). Based on the hypothesis that SSP1 could have an activity related to a Saccharomyces cerevisiae glucanosyl-transferase (Gas1), sequence(s) comparative analysis and functional complementation tests on Δgas1mutant argued against this hypothesis. Biochemical assays revealed a β–glucuronidase activity for SSP1, but the level was pretty low. SSP1 would possess a glycoside-cleavage activity, but with a different substrate. All of the SSP1 sequences, called SSP1-like, have been retrieved in order to study their distribution among fungi. The analysis evidenced that the presence of the gene was not related to the trophic mode. Determination of the role of the protein in wood decay process is still ongoing
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Master thesis
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Submitted on : Thursday, February 7, 2019 - 11:58:12 AM
Last modification on : Saturday, April 30, 2022 - 4:03:10 PM
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  • HAL Id : hal-02010585, version 1



Thomas Bacchetta. Caractérisation fonctionnelle des protéines SSP1-like : approches biochimiques et physiologiques. Biotechnologies. 2018. ⟨hal-02010585⟩



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