Caractérisation fonctionnelle des protéines SSP1-like : approches biochimiques et physiologiques

Abstract : During the wood-decay process, Phanerochaete chrysosporium is submitted to an "extractives" induced stress. Transcriptomic analysis on P. chrysosporium in the presence of wood extracts revealed an induction of genes which encode stress response proteins and small secreted protein with unknown function. The aim of this internship was the characterization of one of these proteins (SSP1). Based on the hypothesis that SSP1 could have an activity related to a Saccharomyces cerevisiae glucanosyl-transferase (Gas1), sequence(s) comparative analysis and functional complementation tests on Δgas1mutant argued against this hypothesis. Biochemical assays revealed a β–glucuronidase activity for SSP1, but the level was pretty low. SSP1 would possess a glycoside-cleavage activity, but with a different substrate. All of the SSP1 sequences, called SSP1-like, have been retrieved in order to study their distribution among fungi. The analysis evidenced that the presence of the gene was not related to the trophic mode. Determination of the role of the protein in wood decay process is still ongoing
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/public/BUS_M_2018_BACCHETTA_THOMAS.pdf
Complete list of metadatas

https://hal.univ-lorraine.fr/hal-02010585
Contributor : Memoires Ul <>
Submitted on : Thursday, February 7, 2019 - 11:58:12 AM
Last modification on : Thursday, February 7, 2019 - 3:03:47 PM
Long-term archiving on : Wednesday, May 8, 2019 - 4:05:39 PM

File

BUS_M_2018_BACCHETTA_THOMAS.pd...
Files produced by the author(s)

Identifiers

  • HAL Id : hal-02010585, version 1

Collections

Citation

Thomas Bacchetta. Caractérisation fonctionnelle des protéines SSP1-like : approches biochimiques et physiologiques. Biotechnologies. 2018. ⟨hal-02010585⟩

Share

Metrics

Record views

17

Files downloads

20