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Comparison of seven methods for extraction of bacterial DNA from fecal and cecal samples of mice

Abstract : Analysis of bacterial DNA from fecal samples of mice is commonly performed in experimental studies. Although DNA extraction is a critical step in various molecular approaches, the efficiency of methods that may be used for DNA extraction from mice fecal samples has never been evaluated. We compared the efficiencies of six widely used commercial kits (MasterPure (TM) Gram Positive DNA Purification Kit, QIAamp (R) DNA Stool Mini Kit; NucliSENS (R) easyMAG (R), ZR Fecal DNA MiniPrep (TM), FastDNA (R) SPIN Kit for Feces and FastDNA (R) SPIN Kit for Soil) and a non-commercial method for DNA isolation from mice feces and cecal contents. DNA quantity and quality were assessed by fluorometry, spectrophotometry, gel electrophoresis and qPCR. Cell lysis efficiencies were evaluated by qPCR targeting three relevant bacteria in spiked specimens. For both feces and intestinal contents, the most efficient extraction method was the FastDNA (R) SPIN Kit for Soil.
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https://hal.univ-lorraine.fr/hal-02314539
Contributor : Jean-Pol Frippiat <>
Submitted on : Saturday, October 12, 2019 - 6:32:40 PM
Last modification on : Tuesday, October 13, 2020 - 10:46:52 AM

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Janina Ferrand, Kevin Patron, Christine Legrand-Frossi, Jean-Pol Frippiat, Christophe Merlin, et al.. Comparison of seven methods for extraction of bacterial DNA from fecal and cecal samples of mice. Journal of Microbiological Methods, Elsevier, 2014, 105, pp.180-185. ⟨10.1016/j.mimet.2014.07.029⟩. ⟨hal-02314539⟩

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