Endophytes are bacteria or fungi that invade living plant tissues without causing disease. As their host plant, endophytes also produce a wide variety of specialized metabolites. At first, we studied the endophytic fungal flora of medicinal plants from Sudan, its molecular characterization was carried out. Then we multiplied the fungus Curvularia papendorfi under controlled conditions. The phytochemical study of extracts of this fungus led to the isolation of 5 biomolecules including a new compound. A set of spectroscopic methods, 1D and 2D NMR, IR, UV and high-resolution mass spectrometry (HR-ESI-MS), were used to determine the structure of this new compound: 3,7,11,15 tetrahydroxy-18-hydroxymethyl-14,16,20,22,24-pentamethylhexacosa-4E, 8E, 12E, 16,18-pentaenoic acid. It showed moderate anti-bacterial activity against S. aureus with the IC50 value = 62.5 μg / mL and low cytotoxicity with the IC50 value > 100 μM against MCF7 cancer cells (human breast carcinoma).