Quantification d’adénovirus infectieux par la détection de marqueurs viraux dans les cellules infectées

Abstract : Adenoviruses are one of the most common enteric viruses responsible for waterborne viral infections. The cell culture assay remains the reference method for detection of infectious viral particles, but it is often slow as it requires at least 10 days of incubation. However, this approach can be associated with viral genome (ICC-qPCR) or viral proteins (immunolabeling in situ) detection methods. Here both methods were assessed for rapid detection and quantification of adenovirus serotype 2 infection. The ICC-qPCR analysis performed at 24 h post-infection allowed the quantification of infectious adenovirus concentrations ranging between 10 and 106 MPNUC/ml (Most Probable Number of Cytopathic Units/mL). At the same time point the immunolabeling in situ led to the detection of infected fluorescent cells at a concentration of 105 MPNUC/ml. We also observed that in the case of evaluation of partial adenovirus inactivation following heat exposure of viral particles (45-55°C), the immunolabeling in situ was the only method remaining consistent with cell culture assay.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/prive/BUS_M_2015_MEO_MARIE.pdf
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https://hal.univ-lorraine.fr/hal-02337088
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Submitted on : Tuesday, October 29, 2019 - 11:49:56 AM
Last modification on : Tuesday, October 29, 2019 - 11:49:56 AM

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Marie Meo. Quantification d’adénovirus infectieux par la détection de marqueurs viraux dans les cellules infectées. Microbiologie et Parasitologie. 2015. ⟨hal-02337088⟩

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