Caractérisation génétique des souches Françaises d’Alternaria associées aux défoliations de pommiers par une approche multilocus (MLST)

Abstract : For a number of years, severe and significant defoliations have been observed in orchards of different apple varieties in the Auvergne Rhône-Alpes region (France). In 2016, sixteen Alternaria strains were isolated from apple leaf samples with necrotic spots and used for genetic characterization using a multilocus approach using Alta1, EndoPG, TMA22, OPA1-3, OPA2-1, and OPA10- 2, already investigated in the framework of several genetic characterization of Alternaria spp. isolated from apple trees. The multilocus analyzes performed revealed that the French strains belong to two clades of the Alternaria section: A. arborescens and A. alternata. The search of the genes responsible for the production of AMT toxins specific to Alternaria alternata f. sp. mali by PCR revealed that none of the French strains would possess these genes. The disease of spots on the leaves and on the fruit of apple trees would be caused by a set of different species of Alternaria.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/ulprive/BUS_M_2017_GNIDE_DOSSI_CARINE.pdf
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https://hal.univ-lorraine.fr/hal-02337716
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Submitted on : Tuesday, October 29, 2019 - 3:47:45 PM
Last modification on : Tuesday, October 29, 2019 - 3:47:46 PM

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  • HAL Id : hal-02337716, version 1

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Citation

Dossi Carine Gnide. Caractérisation génétique des souches Françaises d’Alternaria associées aux défoliations de pommiers par une approche multilocus (MLST). Microbiologie et Parasitologie. 2017. ⟨hal-02337716⟩

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