Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS - Université de Lorraine Access content directly
Journal Articles International Journal of Horticultural Science and Technology Year : 2019

Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS

Abstract

Narcissus spp. are of great importance to pharmaceutical industries because of biosynthesis of Amaryllidaceae alkaloids. N. tazetta with several varieties is a widespread species, native to the Mediterranean region with considerable distribution in Iran. In the present study, calli, roots and bulblets were regenerated from N. tazetta var. Meskin. In order to report alkaloid profile and quantify galanthamine and lycorine, methanolic extract of different in vitro tissues were subjected to alkaloid purification by SPE (solid phase extraction) method, followed by GC-MS analysis. In general, galanthamine and lycorine were detected in all in vitro tissues, while demethylmaritidine was only identified in bulblets. Bulblet was identified as the best source of main Amaryllidaceae alkaloids regarding the highest quantities of galanthamine and lycorine with 15 and 731 µg/g dry weight (DW) respectively.
Fichier principal
Vignette du fichier
Quantitative Determination of Lycorine and Galanthamine in Differentin VitroTissues of Narcissus tazettaby GC-MS.pdf (683.46 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02403509 , version 1 (10-12-2019)

Licence

Attribution

Identifiers

Cite

Ameneh Tarakemeh, Majid Azizi, Vahid Rowshan, Hassan Salehi, Rosella Spina, et al.. Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS. International Journal of Horticultural Science and Technology, 2019, 6, pp.151 - 157. ⟨10.22059/ijhst.2019.280853.295⟩. ⟨hal-02403509⟩
261 View
217 Download

Altmetric

Share

Gmail Facebook X LinkedIn More