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Master thesis

PCR duplex Dermatophytes – T. rubrum sur CFX96® : de la mise au point à l’utilisation en routine

Abstract : Dermatophytes are involved in the majority of fungal infections of the skin, hair and nails. Trichophyton rubrum is the most frequently dermatophyte involved in pathology. Detection and accurate identification of dermatophytes allows the diagnosis to be made and to implement an appropriate treatment. Faster than culture-based identification (the gold standard requiring a considerable expertise), molecular biology techniques are currently preferred. The objective of this internship was to optimize the fungal DNA extraction method (biological samples and cultures) and to develop the detection of dermatophytes and the identification of T. rubrum on CFX96® by a duplex real time PCR. The method comparison showed that this new method is more sensitive than the culture and equally effective than the PCR method previously used by the laboratory.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/ulprive/BUS_M_2019_PABLO_THOMAS.pdf
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https://hal.univ-lorraine.fr/hal-02550345
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Submitted on : Wednesday, April 22, 2020 - 10:19:51 AM
Last modification on : Monday, October 19, 2020 - 2:42:29 PM

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  • HAL Id : hal-02550345, version 1

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Citation

Thomas Pablo. PCR duplex Dermatophytes – T. rubrum sur CFX96® : de la mise au point à l’utilisation en routine. Microbiologie et Parasitologie. 2019. ⟨hal-02550345⟩

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