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Mesenchymal stem cells heterogeneity considering surface markers and senescence

Abstract : Mesenchymal Stem Cells (MSC) hold great potential in the field of regenerative medicine in a wide variety of applications thanks to their self renewal capacity, immunosuppressive properties and paracrine effect. Currently, they are the most used in clinical trials with cell-based treatments. However, even with this important research effort, results remain very heterogeneous. One important challenge with cell therapies is to ensure cell homogeneity and quality with an extensive characterization of their potential. Preliminary studies made in the laboratory have led us toward the hypothesis that MSC gather different cell sub types : different stages of maturation, senescent and younger cells, with specific functionalities. This heterogeneity could induce the difficulty to predict the sample behavior and could be responsible, in part, of the variability of clinical results. The global objective of our study is to understand multi-scale MSC heterogeneity, from the difference between MSC from different sources, to the difference among MSC within one sample. For this, we search for specific surface markers of MSC subtypes and senescent MSC. The final aim is to define functionality biomarkers, sort MSC to concentrate a sub type, eliminate senescent cells and study functionality of the sorted cells. Our study confirmed our hypothesis that there exists high level of heterogeneity among MSC. Difference in surface marker expression can be linked to functional differences observed between MSC from different tissue sources, different donors or from different sub-types. Among the potential markers, CD146 appears as particularly interesting to sort and gather MSC with better potential for cell therapy. CD157 was also identified and associated to aging and replicative senescence. The challenge will be to find a way to obtain a sufficient number of cells. This could be achieved by another strategy consisting in adapting culture conditions to enhance MSC potential and upregulate the functionality markers associated.
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https://hal.univ-lorraine.fr/hal-02973642
Contributor : Naceur Charif <>
Submitted on : Wednesday, October 21, 2020 - 10:39:04 AM
Last modification on : Thursday, October 22, 2020 - 3:27:27 AM

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Laurie Targa, Naceur Charif, Xing Liu, Audrey Fortunat Zeh Eto'O, Mayssa Kacem, et al.. Mesenchymal stem cells heterogeneity considering surface markers and senescence. 8th InternationalSymposium China-Europe Stem cells and regenerative medicine, Jun 2018, Wuhan, China. ⟨hal-02973642⟩

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