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Master thesis

Le virus de l’hépatite E : approche moléculaire de son pouvoir pathogène et de ses modes de circulation entre l’homme et l’environnement

Abstract : Hepatitis E Virus is the principal agent responsible for acute hepatitis worldwide. In developing countries, contamination is linked to faecal peril. In countries with high health standards, HEV genotypes 3 and 4 are the most frequent genotypes found, and contamination is mainly due to ingestion of undercooked or uncooked meat (zoonosis), but could also be linked to the water environment. In France, and more particularly in Lorraine, seroprevalence against HEV is high (28.4% in 2015). Our work consists in analysing the links between viral quasi-species of HEV, infectious power and circulation of strains between humans and environment. For this, we sequenced an area of interest in the HEV overlapping region ORF2/3 by use of two techniques (Sanger sequencing and ultra-deep sequencing), respectively allowing us to highlight the main variant and quasi-species. A prediction of antigenicity according to Parker's model was also performed, in order to analyse impact of protein mutations. Sanger sequencing quickly revealed possible molecular signatures in wild boar strains. Ultra-deep sequencing analysis has allowed hypotheses to be made about the role of water in the circulation of HEV strains between humans and environment. In addition, significant diversity of the quasi-species in humans could reflect greater selection pressure than that observed in animals.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/ulprive/BUS_M_2018_GENTILHOMME_ALEXIS.pdf
Complete list of metadatas

https://hal.univ-lorraine.fr/hal-03024888
Contributor : Thèses Ul <>
Submitted on : Thursday, November 26, 2020 - 9:20:35 AM
Last modification on : Friday, November 27, 2020 - 3:31:10 AM

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  • HAL Id : hal-03024888, version 1

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Alexis Gentilhomme. Le virus de l’hépatite E : approche moléculaire de son pouvoir pathogène et de ses modes de circulation entre l’homme et l’environnement. Biotechnologies. 2018. ⟨hal-03024888⟩

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