Skip to Main content Skip to Navigation
Master thesis

Caractérisation de mutants de Saccharomyces cerevisiae résistants au lanthane et à l’ytterbium obtenus par ingénierie évolutive

Abstract : The current evolution of technologies and population have led to an increasing use of lanthanides such as lanthanum and ytterbium, a light and a heavy rare earth element (REE), respectively. The interactions and mechanisms involved in REE tolerance are poorly studied in eukaryotic organisms. Saccharomyces cerevisiae was used in an evolutionary engineering experiment that consisted in a continuous and increasing exposition to La or Yb to improve its resistance phenotype. This strategy was successful and led to different mutants several dozen times more resistant to La or Yb compared to the wild-type strain. These mutants also acquired cross-resistance to other metals. The genome of the most resistant mutants was sequenced and allowed the identification of different mutations with the corresponding mutated proteins. These proteins belong to functional groups such as calcium transport, protein transport from the endoplasmic reticulum to the plasma membrane, or the endocytosis. In conclusion, this evolutionary engineering approach brings the first data on REE tolerance mechanisms in eukaryotic cells. Several complementary studies have to be done to improved our comprehension and define precisely the identified mechanisms towards REE.
Document type :
Master thesis
Complete list of metadatas

https://hal.univ-lorraine.fr/hal-03024919
Contributor : Thèses Ul <>
Submitted on : Thursday, November 26, 2020 - 9:35:50 AM
Last modification on : Friday, November 27, 2020 - 3:31:10 AM

File

BUS_M_2018_ORY_JORDAN.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : hal-03024919, version 1

Collections

Citation

Jordan Ory. Caractérisation de mutants de Saccharomyces cerevisiae résistants au lanthane et à l’ytterbium obtenus par ingénierie évolutive. Biotechnologies. 2018. ⟨hal-03024919⟩

Share

Metrics

Record views

3

Files downloads

12