Oxidative Effects during Irreversible Electroporation of Melanoma Cells—In Vitro Study - Université de Lorraine Access content directly
Journal Articles Molecules Year : 2020

Oxidative Effects during Irreversible Electroporation of Melanoma Cells—In Vitro Study

Wojciech Szlasa
  • Function : Author
  • PersonId : 1088093
Aleksander Kiełbik
Nina Rembiałkowska
  • Function : Author
  • PersonId : 1088094
Vitalij Novickij
  • Function : Author
  • PersonId : 1088095
Mounir Tarek
Jolanta Saczko
  • Function : Author
  • PersonId : 1088096
Julita Kulbacka
  • Function : Author
  • PersonId : 1088097

Abstract

Irreversible electroporation (IRE) is today used as an alternative to surgery for the excision of cancer lesions. This study aimed to investigate the oxidative and cytotoxic effects the cells undergo during irreversible electroporation using IRE protocols. To do so, we used IRE-inducing pulsed electric fields (PEFs) (eight pulses of 0.1 ms duration and 2–4 kV/cm intensity) and compared their effects to those of PEFs of intensities below the electroporation threshold (eight pulses, 0.1 ms, 0.2–0.4 kV/cm) and the PEFs involving elongated pulses (eight pulses, 10 ms, 0.2–0.4 kV/cm). Next, to follow the morphology of the melanoma cell membranes after treatment with the PEFs, we analyzed the permeability and integrity of their membranes and analyzed the radical oxygen species (ROS) bursts and the membrane lipids’ oxidation. Our data showed that IRE-induced high cytotoxic effect is associated both with irreversible cell membrane disruption and ROS-associated oxidation, which is occurrent also in the low electric field range. It was shown that the viability of melanoma cells characterized by similar ROS content and lipid membrane oxidation after PEF treatment depends on the integrity of the membrane system. Namely, when the effects of the PEF on the membrane are reversible, aside from the high level of ROS and membrane oxidation, the cell does not undergo cell death.
Fichier principal
Vignette du fichier
molecules-26-00154-v2.pdf (2.58 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03104922 , version 1 (10-01-2021)

Licence

Attribution

Identifiers

Cite

Wojciech Szlasa, Aleksander Kiełbik, Anna Szewczyk, Nina Rembiałkowska, Vitalij Novickij, et al.. Oxidative Effects during Irreversible Electroporation of Melanoma Cells—In Vitro Study. Molecules, 2020, 26 (1), pp.154. ⟨10.3390/molecules26010154⟩. ⟨hal-03104922⟩
2723 View
109 Download

Altmetric

Share

Gmail Facebook X LinkedIn More